A Typical DNase I Reaction Protocol (NEO BIOLABS DNASE ENZYME)


A Typical DNase I Reaction Protocol (NEO BIOLABS DNASE ENZYME)

Protocol

  1. Resuspend 10 µg RNA in 1X DNase I Reaction Buffer to a final volume of 100 µl.
  2. Add 2 units of DNase I, mix thoroughly and incubate at 37°C for 10 minutes.
  3. Add 1 µl of 0.5 M EDTA (to a final concentration of 5 mM).
  4. Heat inactivate at 75°C for 10 minutes.
Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s