Vai tro cua SDS trong buffer dung cho lai vi chip


What is the purpose of SDS in microarray hybridization buffers – (Aug/15/2006 )

Does anyone know why SDS is included in hybridization buffers for microarray hybridizations? What purpose does it serve? Thanks

-zpratt-

I do the reverse of micro-array hybridisation –> in situ DNA denaturation. I use a NaOH/SDS denaturant- although the SDS doesn’t denature the DNA, it does reduce non-specific interaction with the surface. It stands to reason the same principle would apply to in situ hybridisation.

-DNA Ligase Ben-

hi
SDS serve as a background reducer during hybidization.
During washes, it serves as gentle wash agent in cooperation with salts (too small quantity for the background matter but still enough for wash).

-fred_33-
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