1 pmol/ul = 1 uM (ghi nhớ đi mày)

Hello Misha,

100pmol/ul is a 100uM solution.(pmol/ul = nmol/ml = umol/L……uM)

If you use 2.5ul of the primer solution into a final volume of 25ul the calculation is as follows.

V1 x C1=V2 x C2 where V= volume in uL, C=concentration in uM

2.5uL x 100uM = 25uL x C2
(2.5 x 100)/25=C2
10 =C2
10uM final primer concenration
(Note: it is important to keep units the same on both sides of =)
It is confusing that 2 of 5 did work, but you might check the MgCl2 concentration in the new Mastermix it may be at a different concentration. Did the 3 samples that failed give any product?? How much DNA do you add?

Hope this helps,

Posts: 32
Joined: Jun 03 2008 12:04 pm
Location: Winnipeg, Manitoba, Canada

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )


Connecting to %s