Dnase 1


DescriptionBack to Top

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Highlights

Isolated from a recombinant source
Supplied with 10X Reaction Buffer

Product Source

An E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
DNase I Reaction Buffer -20 10X

Advantages and FeaturesBack to Top

Applications

  • Degradation of DNA template in transcription reactions
  • Removal of contaminating genomic DNA from RNA samples
  • DNase I footprinting
  • Nick Translation

Properties and UsageBack to Top

Unit Definition

One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.

Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

Reaction Conditions

1X DNase I Reaction Buffer
Incubate at 37°C

1X DNase I Reaction Buffer:
10 mM Tris-HCl
2.5 mM MgCl2
0.5 mM CaCl2
pH 7.6 @ 25°C

Usage Concentration

2,000 units/ml

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
2 mM CaCl2
50% Glycerol
pH 7.6 @ 25°C

Heat Inactivation

75°C for 10 min

Quality ControlBack to Top

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.

  • RNase Activity (1 Hour Digestion):
    The product is tested in a reaction containing a RNA substrate.  After incubation for 1 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

NotesBack to Top

  1. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3).

https://www.neb.com/products/m0303-dnase-i-rnase-free

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