EcoRI


EcoRI

GAATT   C
C   TTAAG

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Frequency of Cutting

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
5 5 0 1 1 1

Incubation Conditions

Buffer H. 37°C.

Percent Activity in 4-CORE® Buffer

A B C D H MULTI-CORE™
25–50% 50–75% 50–75% 50–75% 100% 100%*

*MULTI-CORE™ Buffer is not recommended due to potential star activity.

Source

Escherichia coli RY 13.

Storage Buffer

10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

Storage Conditions

Store at –20°C.

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