- – Store antibodies frozen (-20°C) in lyophilized or concentrated liquid form until needed. Avoid repeated freeze/thaw cycles, which degrade proteins. Instead, freeze reconstituted antibodies in single-use aliquots or add anti-freeze (glycerol or ethylene glycol) so that the stock solution can be maintained at -20°C in liquid form to enable pipetting without “thawing”.
- – Avoid storing antibody (or other protein solutions) for more than a few days at 4°C. Concentrated stocks (> 0.5mg/mL) are usually stable at 4°C for at least 2-3 weeks if they are sterile-filtered and contain anti-microbial additives, such as sodium azide. Do not attempt to store diluted (< 0.1mg/mL) antibody solutions unless stabilized by addition of “carrier” proteins or special additives.
- – Do not use frost-free freezers. These units subject the freezer compartment to regular freeze/thaw cycles to eliminate the frost on the sides of the freezer. These cycles will degrade protein solutions.
For more information, see our list of Tech Tips and related articles in our Protein Methods Library for Thermo Scientific Pierce Protein Research Products. In particular, see Tech Tip #43: Protein Stability and Storage.