p53 Status of Cell Line


http://dna.brc.riken.jp/en/GENESETBANK/211p53p73_2.html

BioResource Center RIKEN BRC DNA BANK

Home Clone set Gene Expression Gene Analysis Search Literature

<trvalign=”bottom”>

Gene Set Collection

Cloned DNA Reporter (target) p53 status p53 mutant

p53, p63 and p73

p53 Status of Cell Line

Cell line Origin Status (link)
A2780 Human ovarian tumour cell p53 status wt, elevated
p14(ARF) protein status +
p53 gene status [ wt ]
Rb gene status [ wt ]
A375 p14(ARF) gene status deficient
p14(ARF) protein status LTD
p53 protein status +
p53 gene status [ wt ]
Rb gene status [ wt ]
A549 p53 gene status wt
p16(INK4) protein LTD
pRb protein +
p14(ARF) protein status LTD
p14(ARF) gene status +
p14(ARF) protein status LTD
p53 gene status [ wt ]
Rb gene status [ wt ]
ATN-1 p53 gene status [ wt (RCB1440) ]
Rb gene status [ wt (RCB1440) ]
BALL-1 p53 gene status [ Asp281Gly (RCB0256) ]
Rb gene status [ wt (RCB0256) ]
Bre80 p14(ARF) gene status +
p14(ARF) protein status +
p53 gene status wt
BT-549 Human breast carcinoma p16(INK4) protein +++++
p16(INK4) protein +
pRb protein LTD
p53 gene status 
p53 gene status [ Arg249Ser ]
Rb gene status [ deletion in coding region ]
C33A p53 gene status Mutant, Arg273Cys
p53 gene status Mutant, Arg273Cys
HPV genome 
p16(INK4) protein +
pRb protein LTD
p53 gene status +/Mutant
p14(ARF) gene status +
p14(ARF) protein status +
p53 gene status [ Arg273Cys ]
Rb gene status [ mutation in an intron ]
CHP-126 p53 gene status [ wt (RCB0486) ]
Rb gene status [ wt (RCB0486) ]
CHP-134 p53 gene status [ wt (RCB0487) ]
Rb gene status [ wt (RCB0487) ]
COLO 679 p53 gene status [ wt (RCB0989) ]
Rb gene status [ wt (RCB0989) ]
CTB-1 p53 gene status [ wt (RCB1316) ]
Rb gene status [ wt (RCB1316) ]
CW-2 p53 gene status [ wt (RCB0778) ]
Rb gene status [ wt (RCB0778) ]
ECC10 p53 gene status [ wt (RCB0983) ]
Rb gene status [ Gln685stop (RCB0983) ]
ECC12 p53 gene status [ Val216Met (RCB1009) ]
Rb gene status [ deletion (RCB1009) ]
ECC4 p53 gene status [ deletion (RCB0982) ]
Rb gene status [ Arg661Trp (RCB0982) ]
EC-GI-10 p53 gene status [ Tyr234Cys, Arg273Leu (RCB0774) ]
Rb gene status [ wt (RCB0774) ]
EoL-1 cell p53 gene status [ wt (RCB0641) ]
Rb gene status [ wt (RCB0641) ]
ETK-1 p53 gene status [ Arg175His (RCB1289) ]
Rb gene status [ wt (RCB1289) ]
G401 p53 status wt
p14(ARF) protein status LTD
p53 gene status [ wt ]
Rb gene status [ wt ]
GCIY p53 gene status [ Glu11Gln, His179Gln (RCB0555) ]
Rb gene status [ wt (RCB0555) ]
GI-1 p53 gene status [ Cys275Tyr (RCB0763) ]
Rb gene status [ Arg552stop (RCB0763) ]
GM 639 DM p14(ARF) gene status +
p14(ARF) protein status +
p53 gene status wt/LT
GM 847 DM p14(ARF) gene status +
p14(ARF) protein status +
p53 gene status wt/LT
GOTO-P3 p53 gene status [ wt (RCB0721) ]
Rb gene status [ wt (RCB0721) ]
GR-ST p53 gene status [ Met246Thr (RCB1182) ]
Rb gene status [ mutation in an intron (RCB1182) ]
H1299 p53 gene status Null, deletion
p14(ARF) protein status +++
p53 gene status Null
p14(ARF) gene status +
p14(ARF) protein status ++
HAL-01 p53 gene status [ wt (RCB0540) ]
Rb gene status [ wt (RCB0540) ]
HCE-T p53 gene status [ wt (RCB1384) ]
Rb gene status [ wt (RCB1384) ]
HCT116 p53 gene status wt
p16(INK4) protein LTD
pRb protein +
p53 status wt
p14(ARF) protein status LTD
p53 protein status +
p53 gene status [ wt ]
Rb gene status [ wt ]
HCT116 TP53 -/- p53 status KO
p14(ARF) protein status LTD
HDF p14(ARF) gene status +
p14(ARF) protein status LTD
p53 gene status wt
HEK293 Human embryonic kidney p53 gene status wt/E1b55k
pRb genotype +/E1A
p16(INK4) protein +
pRb protein +/E1A
p16(INK4) protein ++++
p14(ARF) gene status +
p14(ARF) protein status +
p53 gene status wt/E1b55k
HeLa Human cervical carcinoma HPV genome HPV-18/+
p53 gene status wt/E6
pRb genotype +/E7
p16(INK4) protein ++++
p14(ARF) gene status +
p14(ARF) protein status ++
p53 gene status wt/E6
H-EMC-SS p53 gene status [ wt (RCB0508) ]
Rb gene status [ wt (RCB0508) ]
Hep3B Human hepatoma p53 gene status partial deletion
p53 protein status LTD
P16 genotype +
p16(INK4) protein +
pRb protein LTD
p53 gene status -/deficient
HepG2 Human hepatoma p53 gene status wt (Southern blott)
p53 protein status +
P16 genotype +
p16(INK4) protein +
pRb protein +
p14(ARF) gene status +
p14(ARF) protein status LTD
HGC-27 p53 gene status [ flameshift after Pro153 (RCB0500) ]
Rb gene status [ wt (RCB0500) ]
HMV-II p53 gene status [ Asn268Ile (RCB0777) ]
Rb gene status [ wt (RCB0777) ]
HT29 p53 gene status Mutant, Arg273His
p16(INK4) protein LTD
pRb protein +
p16(INK4) protein LTD
p53 protein status +
pRb protein +
p14(ARF) protein status +
p14(ARF) protein status ++
p53 gene status [ Arg273His ]
Rb gene status [ wt ]
HTC/C3 p53 gene status [ Pro152Leu (RCB0452) ]
Rb gene status [ wt (RCB0452) ]
HuH-7 Human hepatoma p53 gene status Mutant, Tyr220Cys
p16(INK4) protein LTD
p53 protein status +, truncated
pRb protein +
p53 gene status [ Tyr220Cys (RCB1366) ]
Rb gene status [ wt (RCB1366) ]
IA-LM p53 gene status [ wt (RCB0554) ]
Rb gene status [ wt (RCB0554) ]
IIICF/c p14(ARF) gene status 
p14(ARF) protein status LTD
p53 gene status Null
J82 p14(ARF) gene status +
p14(ARF) protein status ++
p53 gene status Mutant
p53 gene status [ Lys320Asn, deletion in coding region ]
Rb gene status [ mutation in an intron ]
KGN p53 gene status [ wt (RCB1154) ]
Rb gene status [ wt (RCB1154) ]
KP4 p53 gene status [ wt (RCB1005) ]
Rb gene status [ wt (RCB1005) ]
KU812 p53 gene status
p53 gene status [ wt (RCB0495) ]
Rb gene status [ wt (RCB0495) ]
LC-1F p53 gene status [ Met237Ile (RCB0439) ]
Rb gene status [ wt (RCB0439) ]
LC-2/ad p53 gene status [ deletion in coding region (RCB0440) ]
Rb gene status [ wt (RCB0440) ]
LOVO Human colon carcinoma p16(INK4) protein LTD
p53 protein status LTD
pRb protein +
p16(INK4) protein LTD
p53 protein status LTD
pRb protein +
p53 gene status [ wt ]
Rb gene status [ wt ]
LS 174 T Human colon carcinoma p16(INK4) protein LTD
pRb protein +
p53 gene status wt
p53 gene status [ wt ]
Rb gene status [ wt ]
Lu-134-A p53 gene status [ Pro278Leu (RCB0466) ]
Rb gene status [ Try75stop (RCB0466) ]
Lu-135 p53 gene status [ Gly244Cys (RCB0468) ]
Rb gene status [ flame shift after Arg661 (RCB0468) ]
Lu-139 p53 gene status [ Val157Phe (RCB0469) ]
Rb gene status [ flame shift after Gly617 (RCB0469) ]
Lu-165 p53 gene status [ deletion in an intron (RCB1184) ]
Rb gene status [ flame shift after Val157 (RCB1184) ]
MCF-7 Human breast carcinoma p16(INK4) protein LTD
pRb protein +
p16(INK4) protein LTD
pRb protein +
p14(ARF) protein status LTD
p53 protein status +
p53 status +/wt
p53 gene status wt
p53 gene status [ wt ]
Rb gene status [ wt ]
MD468 p53 gene status +/Mutant
MDA-MB-453 p53 gene status [ wt (RCB1192) ]
Rb gene status [ wt (RCB1192) ]
MFH-ino p53 gene status [ wt (RCB0749) ]
Rb gene status [ wt (RCB0749) ]
MKN28 p53 gene status [ Ile251Leu (RCB1000) ]
Rb gene status [ wt (RCB1000) ]
MKN45 p53 gene status Arg110Cys
p53 gene status [ wt (RCB1001) ]
Rb gene status [ wt (RCB1001) ]
MMAc-SF p53 gene status [ wt (RCB1200) ]
Rb gene status [ wt (RCB1200) ]
NB16 p53 gene status [ Arg175His (RCB0478) ]
Rb gene status [ wt (RCB0478) ]
NB69 p53 gene status [ wt (RCB0480) ]
Rb gene status [ wt (RCB0480) ]
NBsusSR p53 gene status [ wt (RCB0803) ]
Rb gene status [ wt (RCB0803) ]
NEC8 p53 gene status [ wt (RCB0489) ]
Rb gene status [ wt (RCB0489) ]
NOS-1 p53 gene status [ Arg273His (RCB1032) ]
Rb gene status [ wt (RCB1032) ]
OCUB-M p53 gene status [ Gly244Ser (RCB0881) ]
Rb gene status [ flame shift after Ile42 (RCB0881) ]
OMC-1 p53 gene status [ wt (RCB0753) ]
Rb gene status [ wt (RCB0753) ]
OS-RC-2 p53 gene status [ wt (RCB0735) ]
Rb gene status [ wt (RCB0735) ]
PC-14 p53 gene status [ Arg248Gln (RCB0446) ]
Rb gene status [ wt (RCB0446) ]
RCC10RGB p53 gene status [ wt (RCB1151) ]
Rb gene status [ wt (RCB1151) ]
RKO p14(ARF) gene status +
p14(ARF) protein status LTD
p53 gene status wt
p53 gene status [ wt ]
Rb gene status [ wt ]
RKO p53.13 p14(ARF) gene status +
p14(ARF) protein status LTD
p53 gene status wt/DN
Saos 2 p14(ARF) gene status +
p14(ARF) protein status +
p14(ARF) protein status +
p16(INK4) protein +/-
p53 gene status Null
p53 gene status deletion coding region
p53 protein status LTD
pRb protein +/truncated
p53 gene status [ wt ]
Rb gene status [ deletion in coding region ]
T98G p14(ARF) protein status LTD
pRb protein +
p14(ARF) gene status 
p14(ARF) protein status LTD
p53 gene status Mutant
p53 gene status [ Met237Ile ]
Rb gene status [ wt ]
TC-YIK p53 gene status [ deletion in coding region (RCB0443) ]
Rb gene status [ wt (RCB0443) ]
TGBC11TKB p53 gene status [ Arg273Cys, flame shift after Lys382 (RCB1148) ]
Rb gene status [ wt (RCB1148) ]
TGBC1TKB p53 gene status [ flame shift after Cys176 (RCB1129) ]
Rb gene status [ wt (RCB1129) ]
TGBC24TKB p53 gene status [ flame shift after Cys242 (RCB1196) ]
Rb gene status [ wt (RCB1196) ]
TIG3 p14(ARF) protein status LTD
U2OS osteosarcoma p53 gene status wt
p53 protein status +
p16(INK4) protein LTD
p14(ARF) protein status LTD
p53 gene status wt
p14(ARF) protein status LTD
p14(ARF) gene status +
p14(ARF) protein status LTD
p53 gene status [ wt ]
Rb gene status [ wt ]
U373 glioblastoma p53 gene status negative, codon 273 mutation
U87 glioblastoma p53 gene status wt
p14(ARF) protein status LTD
WI-38 normal fibloblast p16(INK4) protein +
p16(INK4) protein +
pRb protein +

LTD, less than detection.
Data in [ ], from Cancer Cell Line Project, COSMIC, Wellcome Trust Sanger Institute. (http://www.sanger.ac.uk/genetics/CGP/cosmic/)

Reference

  • Bressac B, Galvin KM, Liang TJ, Isselbacher KJ, Wands JR, Ozturk M. Abnormal structure and expression of p53 gene in human hepatocellular carcinoma. Proc Natl Acad Sci U S A. 87 (5): 1973-1977, 1990.
  • Bunz F, Dutriaux A, Lengauer C, Waldman T, Zhou S, Brown JP, Sedivy JM, Kinzler KW, Vogelstein B. Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science. 282 (5393): 1497-501.
  • Crook T, Wrede D, Vousden KH. p53 point mutation in HPV negative human cervical carcinoma cell lines. Oncogene 6 (5): 873-875, 1991.
  • Diller L, Kassel J, Nelson CE, Gryka MA, Litwak G, Gebhardt M, Bressac B, Ozturk M, Baker SJ, Vogelstein B, et al. p53 functions as a cell cycle control protein in osteosarcomas. Mol Cell Biol. 1990 Nov;10(11):5772-5781.
  • Edwards SJ, Dix BR, Myers CJ, Dobson-Le D, Huschtscha L, Hibma M, Royds J, Braithwaite AW. Evidence that replication of the antitumor adenovirus ONYX-015 is not controlled by the p53 and p14(ARF) tumor suppressor genes. J Virol. 2002 Dec;76(24):12483-90.
  • Goodrum FD, Ornelles DA. p53 status does not determine outcome of E1B 55-kilodalton mutant adenovirus lytic infection. J. Virol. 72 (12): 9479-9490, 1998.
  • GRAHAM, F.L., SMILEY, J., RUSSELL, W.C., and NAIRN, R. (1977). Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59.74.
  • Greenblatt MS, Bennett WP, Hollstein M, Harris CC. Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res. 54 (18): 4855-4878, 1994.
  • Higashi, H., I. Suzuki-Takahashi, E. Yoshida, S. Nishimura, and M. Kitagawa. 1997. Expression of p16INK4a suppresses the unbounded and anchorage- independent growth of a glioblastoma cell line that lacks p16INK4a. Biochem. Biophys. Res. Commun. 231:743.750.
  • Hsu IC, Tokiwa T, Bennett W, Metcalf RA, Welsh JA, Sun T, Harris CC. p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines. Carcinogenesis 4 (5): 987-992, 1993.
  • Huschtscha, L. I., J. R. Noble, A. A. Neumann, E. L. Moy, P. Barry, J. R. Melki, S. J. Clark, and R. R. Reddel. 1998. Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells. Cancer Res. 58:3508-3512.
  • Jia LQ, Osada M, Ishioka C, Gamo M, Ikawa S, Suzuki T, Shimodaira H, Niitani T, Kudo T, Akiyama M, Kimura N, Matsuo M, Mizusawa H, Tanaka N, Koyama H, Namba M, Kanamaru R, Kuroki T. Screening the p53 status of human cell lines using a yeast functional assay. Mol. Carcinog. 19 (4): 243-253, 1997.
  • Kessis TD, Slebos RJ, Nelson WG, Kastan MB, Plunkett BS, Han SM, Lorincz AT, Hedrick L, Cho KR. Human papillomavirus 16 E6 expression disrupts the p53-mediated cellular response to DNA damage. Proc Natl Acad Sci U S A. 90 (9): 3988-3992, 1993.
  • Lehman TA, Bennett WP, Metcalf RA, Welsh JA, Ecker J, Modali RV, Ullrich S, Romano JW, Appella E, Testa JR, et al. p53 mutations, ras mutations, and p53-heat shock 70 protein complexes in human lung carcinoma cell lines. Cancer Res. 51 (15): 4090-4096, 1991.
  • Masuda H, Miller C, Koeffler HP, Battifora H, Cline MJ. Rearrangement of the p53 gene in human osteogenic sarcomas. Proc Natl Acad Sci U S A. 84 (21): 7716-7719, 1987.
  • Mitsudomi T, Steinberg SM, Nau MM, Carbone D, D’Amico D, Bodner S, Oie HK, Linnoila RI, Mulshine JL, Minna JD, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7 (1): 171-180, 1992.
  • OKAMOTO, A., DEMETRICK, D.J., SPILLARE, E.A., HAGIWARA, K., HUSSAIN, S.P., BENNETT, W.P., FORRESTER, K., GERWIN, B., SERRANO, M., and BEACH, D.H. (1994). Mutations and altered expression of p16INK4 in human cancer. Proc. Natl. Acad. Sci. U.S.A. 91, 11045.11049.
  • Ries SJ, Brandts CH, Chung AS, Biederer CH, Hann BC, Lipner EM, McCormick F, Korn WM. Loss of p14ARF in tumor cells facilitates replication of the adenovirus mutant dl1520 (ONYX-015). Nat Med. 2000 Oct;6(10):1128-1133.
  • Rodrigues NR, Rowan A, Smith ME, Kerr IB, Bodmer WF, Gannon JV, Lane DP. p53 mutations in colorectal cancer. Proc Natl Acad Sci U S A. 87 (19): 7555-7559, 1990.
  • Rogan EM, Bryan TM, Hukku B, Maclean K, Chang AC, Moy EL, Englezou A, Warneford SG, Dalla-Pozza L, Reddel RR. Alterations in p53 and p16INK4 expression and telomere length during spontaneous immortalization of Li-Fraumeni syndrome fibroblasts. Mol Cell Biol. 1995 Sep;15(9):4745-4753.
  • Rothmann T, Hengstermann A, Whitaker NJ, Scheffner M, zur Hausen H. Replication of ONYX-015, a potential anticancer adenovirus, is independent of p53 status in tumor cells. J. Virol. 72 (12): 9470-9478, 1998.
  • Sandig V, Brand K, Herwig S, Lukas J, Bartek J, Strauss M. Adenovirally transferred p16INK4/CDKN2 and p53 genes cooperate to induce apoptotic tumor cell death. Nat Med. 3 (3): 313-319, 1997.
  • Scheffner M, M?nger K, Byrne JC, Howley PM. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc Natl Acad Sci U S A. 88 (13): 5523-5527, 1991.
  • SCHNEIDER-GADICKE, A., and SCHWARZ, E. (1986). Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5, 2285.2292.
  • Steegenga WT, Riteco N, Bos JL. Infectivity and expression of the early adenovirus proteins are important regulators of wild-type and DeltaE1B adenovirus replication in human cells. Oncogene 18 (36): 5032-5043, 1999.
  • Stott FJ, Bates S, James MC, McConnell BB, Starborg M, Brookes S, Palmero I, Ryan K, Hara E, Vousden KH, Peters G. The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2.EMBO J. 1998 Sep 1;17(17):5001-5014.
  • Tam SW, Shay JW, Pagano M. Differential expression and cell cycle regulation of the cyclin-dependent kinase 4 inhibitor p16Ink4. Cancer Res. 54 (22): 5816-5820, 1994.
  • Tominaga O, Nita ME, Nagawa H, Fujii S, Tsuruo T, Muto T. Expressions of cell cycle regulators in human colorectal cancer cell lines. Jpn J Cancer Res. 88 (9): 855-860, 1997.
  • Turnell AS, Grand RJ, Gallimore PH. The replicative capacities of large E1B-null group A and group C adenoviruses are independent of host cell p53 status. J. Virol. 73 (3): 2074-2083, 1999.
  • Ullrich SJ, Sakaguchi K, Lees-Miller SP, Fiscella M, Mercer WE, Anderson CW, Appella E. Phosphorylation at Ser-15 and Ser-392 in mutant p53 molecules from human tumors is altered compared to wild-type p53. Proc Natl Acad Sci U S A. 90 (13): 5954-5958, 1993.
  • Waldman T, Kinzler KW, Vogelstein B. p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Cancer Res. 55 (22): 5187-5190, 1995.
  • Whitaker NJ, Bryan TM, Bonnefin P, Chang AC, Musgrove EA, Braithwaite AW, Reddel RR. Involvement of RB-1, p53, p16INK4 and telomerase in immortalisation of human cells. Oncogene 11 (5): 971-976, 1995.

 go to Head


2011.10.01


Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s